{"id":595,"date":"2020-12-04T16:57:32","date_gmt":"2020-12-04T14:57:32","guid":{"rendered":"https:\/\/cernesim.uaic.ro\/?page_id=595"},"modified":"2020-12-22T21:36:33","modified_gmt":"2020-12-22T19:36:33","slug":"l2-equipment","status":"publish","type":"page","link":"https:\/\/cernesim.uaic.ro\/index.php\/language\/en\/l2-en\/l2en\/l2-equipment\/","title":{"rendered":"L2 &#8211; Equipment"},"content":{"rendered":"<p><img loading=\"lazy\" class=\"size-full wp-image-1485 alignright\" src=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/camera_crestere_plante-222x300-1.jpg\" alt=\"\" width=\"222\" height=\"300\" \/><strong>Climate chamber,<\/strong> <strong>Snijders Scientific, The Netherlands<\/strong><\/p>\n<p>The climate chamber is designed to be used for a wide range of research applications like seed germination, protoplasm cultivation, tissue culture, plant growth, Arabidopsis tests, celular division and many other within life sciences with the possibility to imitate the natural environmental conditions.<\/p>\n<p>With a total volume of 432 liters, as well as a usable area\/shelf of 0.36 m<sup>2\u00a0<\/sup>, the chamber allows to use of an increased number of experimental replicates. The parameters that can be configured are: temperature (between +5 and +40\u00b0C), light intensity (between 0 and 10.000 Lx), humidity (between 5 and 100%). The cycle programming allows experimental standardization; up to 50 programs, as well as up to 99 segments\/program.<\/p>\n<p><strong>Thermal imaging equipment Lemnatec Scanalyzer PL, LemnaTec GmbH, Germany<\/strong><\/p>\n<p><img loading=\"lazy\" class=\" wp-image-1167 alignright\" src=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/echipament_imagistica-300x229.jpg\" alt=\"\" width=\"254\" height=\"194\" srcset=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/echipament_imagistica-300x229.jpg 300w, https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/echipament_imagistica.jpg 328w\" sizes=\"(max-width: 254px) 100vw, 254px\" \/><\/p>\n<p>The system allows the analysis of the organisms&#8217; development phenotypic parameters in non-destructive conditions. This process is possible by using video and IR sensors placed above the item.\u00a0Also, a double lighting system (top, back) ensures the necessary\u00a0conditions for performing analyzes. Captures are made at a frame rate of 3.75 &#8211; 7.5 fps, allowing real-time observations. The categories of organisms that can be traced are varied; from microorganisms (bacteria, fungi) grown in Petri dishes to higher plant organisms (plants) grown in large containers. In these organisms, different processes can be quantified, such as the accumulation of biomass or the evolution of the leaf surface.<\/p>\n<p><strong>Kjeldahl VELP System for the determination of total nitrogen and protein<\/strong><\/p>\n<p><img loading=\"lazy\" class=\"size-medium wp-image-1169 alignright\" src=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/VELP-300x228.jpg\" alt=\"\" width=\"300\" height=\"228\" srcset=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/VELP-300x228.jpg 300w, https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/VELP.jpg 526w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>The system consists of three complementary units for the determination of total nitrogen content from\u00a0mineral, microbial, vegetable or animal sources, having the ability to use liquid or solid samples.\u00a0<span lang=\"en\">The DK6 Velp Scientifica digestion u<\/span><span lang=\"en\">nit co<\/span><span lang=\"en\">nsists of an <\/span><span lang=\"en\">electronically controlled 6-station aluminum block. The temperature of th<\/span><span lang=\"en\">e block can be <\/span><span lang=\"en\">adju<\/span><span lang=\"en\">sted between<\/span><span lang=\"en\"> 45-450\u00b0C, through 20 programs and 4\u00a0 temperature and time ramps. The fume neutralization system (SMS scrubber) coupled with the water recirculation pump (JP) ensures the condensation and neutralization of fumes from the digestion process. <\/span><span lang=\"en\">The UDK 139 Semi-Automatic Kjeldahl Distillation Unit allows a rapid distillation of the digested solutions. <\/span><span lang=\"en\">The unit allows the scheduling of distillation time, automatic addition of sodium hydroxide and water, also the emptying of the tube. <\/span><span lang=\"en\">The degree of nitrogen recovery is over 99.5%. The distillation rate is 35-40 ml\/min.<\/span><\/p>\n<p><strong>Leica DM750 optical microscope<\/strong><\/p>\n<p style=\"text-align: left\">The microscope is provided with 4 objective lens:<\/p>\n<ul>\n<li>achromatic plane 4x minimum aperture 0.10<img loading=\"lazy\" class=\"wp-image-1170 alignright\" src=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/DM750-294x300.jpg\" alt=\"\" width=\"248\" height=\"253\" srcset=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/DM750-294x300.jpg 294w, https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/DM750.jpg 326w\" sizes=\"(max-width: 248px) 100vw, 248px\" \/><\/li>\n<li>achromatic plane 10x minimum aperture 0.22<\/li>\n<li>achromatic plane 40x minimum aperture 0.65<\/li>\n<li>semi-apochromatic plane with oill immersion 100x minimum aperture 1.30<\/li>\n<\/ul>\n<p>The trinocular tube allows the installation of a 12 Mp DFC500 digital camera for microscopy to capture images. <span lang=\"en\">The microscope is equipped with 10x achromatic plane eyepieces and 5mm\/0.5 mm reticles. It has an Abbe capacitor, with an aperture of 1, 25. The field aperture is adjustable for Koehler lighting. Also, i<\/span><span lang=\"en\">t has a 0.63 x C-mount adapter for digital camera with sensor.<\/span><\/p>\n<p><strong><span lang=\"en\">Leica M205A stereo microscope with DFC500 camera<\/span><\/strong><\/p>\n<p style=\"text-align: left\">The microscope is equipped with automatic motorization, for examination in transmitted and\u00a0incident light. <span lang=\"en\">The purpose of this de<\/span><img loading=\"lazy\" class=\" wp-image-1173 alignright\" src=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/M205A-300x293.jpg\" alt=\"\" width=\"254\" height=\"248\" srcset=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/M205A-300x293.jpg 300w, https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/M205A.jpg 341w\" sizes=\"(max-width: 254px) 100vw, 254px\" \/><span lang=\"en\">vic<\/span><span lang=\"en\">e is to observe the surface structures, allow<\/span>ing a magnification ratio of 20.5: 1 and a Zoom range of\u00a07.8x-160x. 1.0x and 2.0x Planapochromat lenses allow a maximum magnification of 256x and 512x, respectively, with 16x eyepieces. The motorized column has a height of 620 mm, provided with motorized zoom and a motorized diaphragm for the field depth. The light source for the transmitted light is a 20W halogen bulb and a set of adjustable mirrors for dark field lighting and oblique lighting (phase contrast). Incident lighting source with 20W fiber optics. Camera resolution: 12 MPxl.<\/p>\n<p style=\"text-align: left\"><strong><span lang=\"en\">Transmission Electron Microscope (TEM) FEI Tecnai G2 Spirit BioTWIN<\/span><\/strong><\/p>\n<p>The Tecnai G2 Spirit BioTWIN microscope allows the analysis of biological samples from animal\u00a0<img loading=\"lazy\" class=\"size-medium wp-image-1175 alignright\" src=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/TEMBioTwin-231x300.jpg\" alt=\"\" width=\"231\" height=\"300\" srcset=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/TEMBioTwin-231x300.jpg 231w, https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/TEMBioTwin.jpg 376w\" sizes=\"(max-width: 231px) 100vw, 231px\" \/>and plant tissues, also from organic and inorganic samples, being one of the basic research tools in the field of biology, medicine and cellular and molecular pathology. <span lang=\"en\">Guaranteed line r<\/span><span lang=\"en\">esolution: 0.34 nm; guaranteed point resolution: 0.<\/span><span lang=\"en\">49 n<\/span><span lang=\"en\">m; maximum acceleration voltage of 120 kV; high voltages can be adjusted continuously or in 10 V steps in the range of 20 &#8211; 120 kV via the Graphical User Interface; the changing of the high voltage is done by software, in less than 1 minute; the stability of high voltage is better than 2 ppm\/min. <\/span><span lang=\"en\">Calculated at the maximum acceleration voltage, the stability is 0.24 V\/min. <\/span><span lang=\"en\">Magnification on the fluorescent screen in the range 22x &#8211; 340000x, continuous or in steps variable; the modification of the magnification is done without the rotation of the image obtained on the area of \u200b\u200binterest (rotation free imaging). The c<\/span><span lang=\"en\">hanging of the analyzed specimen is done in less than 30 seconds by keeping the vacuum in the column and without interrupting the high voltage or emission.<\/span><\/p>\n<p><strong>HansaTech LeafLab photosynthetic\/respiratory performance determination system<\/strong><\/p>\n<p><span lang=\"en\">The system is used for the study of photosynthesis and for respiration measurements by illuminating the <img loading=\"lazy\" class=\"size-medium wp-image-1176 alignright\" src=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/LeafLab-300x221.jpg\" alt=\"\" width=\"300\" height=\"221\" srcset=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/LeafLab-300x221.jpg 300w, https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/LeafLab.jpg 404w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>samples in the gaseous phase, being able to analyze leaves, algae, mosses or samples cut from leaves, with an area of \u200b\u200bup to 10 cm<sup>2<\/sup>. <\/span><span lang=\"en\">The control unit allows a wide range of applications such as mitochondrial and cellular respiration analysis, photosynthesis in chloroplast suspensions or foliar photosynthesis. <\/span><span lang=\"en\">Oxygen measurement is performed in the range of 0-40%, with a minimum resolution of 10 \u00d7 10-5 \u03bcmol\/ml at 20\u00b0C. <\/span><span lang=\"en\">The measuring chamber is provided with an additional optical port for the simultaneous determination of chlorophyll fluorescence. <\/span><span lang=\"en\">The light source consists of 36 red LEDs with a maximum wavelength of 650 nm.<\/span><\/p>\n<p><strong><span lang=\"en\">HansaTech ChloroLab3 Photosynthetic\/Respiratory Performance Determination System<\/span><\/strong><\/p>\n<p><span lang=\"en\">The system is used for the study of photosynthesis and measurements of respiration by illuminating<img loading=\"lazy\" class=\" wp-image-1177 alignright\" src=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/ChloroLab3.jpg\" alt=\"\" width=\"167\" height=\"217\" \/> samples in the liquid phase (algae, seagrass) in volumes up to 20 ml. <\/span><span lang=\"en\">The control unit is specially designed for determining the oxygen absorption and its evolution in the range 0-40%, with a minimum resolution of 10\u00d710<sup>-6<\/sup> \u03bcmol\/ml, at a polarization voltage of 700 mV, being also provided with a magnetic stirrer, which ensures a speed range between 150-900 rpm and a reading acquisition rate of 0.2-10 readings\/sec. <\/span><span lang=\"en\">The measurement of the incident light and the temperature inside the chamber can be performed in a range of 0-50,000 \u03bcmol\/m2\/s, in the range of 400-700 nm and between 0-50\u00b0C.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><strong>HansaTech FMS2 Portable fluorimeter for chlorophyll\u00a0<\/strong><\/p>\n<p><span lang=\"en\">It is a pulse modulation fluorimeter that can be used to measure chlorophyll fluorescence emissions<img loading=\"lazy\" class=\"size-medium wp-image-1179 alignright\" src=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/FMS2-300x219.jpg\" alt=\"\" width=\"300\" height=\"219\" srcset=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/FMS2-300x219.jpg 300w, https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/FMS2.jpg 401w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/> in a wide range of plant material during the photosynthesis process, in ambient lighting conditions (in the field). <\/span><span lang=\"en\">The light sources are represented by a modulated beam with a wavelength of 594 nm, with temperature compensation and 4 frequency control steps, a halogen dualscope lamp with actinic light (0-3000 \u03bcmol\/m<sup>2<\/sup>\/s in 50 steps) and pulse saturation (0-20000 \u03bcmol\/m<sup>2<\/sup>\/s in 100 steps) and 735 nm LED source for PSI preferential excitation. <\/span><span lang=\"en\">The detection is performed by analyzing the peaks of the fast pulses, at a sampling frequency of 10-20Hz, with the received data and the calculated parameters, drawing real-time graphs.\u00a0<\/span><\/p>\n<p><strong>Leica RM2245 semi-automatic microtome<img loading=\"lazy\" class=\" wp-image-1180 alignright\" src=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/RM2245-229x300.jpg\" alt=\"\" width=\"171\" height=\"224\" srcset=\"https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/RM2245-229x300.jpg 229w, https:\/\/cernesim.uaic.ro\/tmp\/files\/2020\/12\/RM2245.jpg 258w\" sizes=\"(max-width: 171px) 100vw, 171px\" \/><\/strong><\/p>\n<p><span lang=\"en\">The Leica RM2245 semi-automatic microtome is used for making paraffin sections for histology and anatomy observations. The sectioning can be done in the conventional or \u201crocking\u201d mode, the device being provided with support for histological cassettes, two wheel locking systems with handle and removable control panel, which allows the control of all the microtome functions. Shaping thickness range: 1-600 \u03bcm. Range of sectioning thicknesses: 0.5-100\u03bcm.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Climate chamber, Snijders Scientific, The Netherlands The climate chamber is designed to be used for a wide range of research applications like seed germination, protoplasm cultivation, tissue culture, plant growth, Arabidopsis tests, celular division and many other within life sciences with the possibility to imitate the natural environmental conditions. With a total volume of 432 [&hellip;]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":585,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/cernesim.uaic.ro\/index.php\/wp-json\/wp\/v2\/pages\/595"}],"collection":[{"href":"https:\/\/cernesim.uaic.ro\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/cernesim.uaic.ro\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/cernesim.uaic.ro\/index.php\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/cernesim.uaic.ro\/index.php\/wp-json\/wp\/v2\/comments?post=595"}],"version-history":[{"count":18,"href":"https:\/\/cernesim.uaic.ro\/index.php\/wp-json\/wp\/v2\/pages\/595\/revisions"}],"predecessor-version":[{"id":1498,"href":"https:\/\/cernesim.uaic.ro\/index.php\/wp-json\/wp\/v2\/pages\/595\/revisions\/1498"}],"up":[{"embeddable":true,"href":"https:\/\/cernesim.uaic.ro\/index.php\/wp-json\/wp\/v2\/pages\/585"}],"wp:attachment":[{"href":"https:\/\/cernesim.uaic.ro\/index.php\/wp-json\/wp\/v2\/media?parent=595"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}